Sephadex:高分辨率首选
目前分辨率、选择性高的凝胶过滤介质,流速快而反压低;
低非特异性吸附,提高回收率
化学物理稳定性高,在0.1M HCl或1M NaOH中40℃ 400h,分辨率保持不变;
装柱方法简单方便,可自行装入实验室用Tricorn、HR、XK柱,生产用BPG柱等层析柱;
Sephadex LH:
极好地分离脂类、激素、维生素和其他小分子量生物分子;
设计适用于在极性有机溶剂和水溶剂混合液中使用;
常用于分离中药;
Sepharose CL:有机溶剂纯化
Sepharose和2,3二溴丙醇反应而成,增强了Sepharose的物理和化学稳定性;
特别适合含有有机溶剂的分离,能承受较强的在位清洗,并可以高温消毒
流速方面比较传统的Sepharose有明显提高。
Sepharose:传统大分子分离
传统的偶联琼脂糖介质,非特异性吸附极低,回收率高;
有三种不同浓度的琼脂糖供选择,分离范围十分宽广,从10,000到40,000,000,适合分离分子量大小差异大,而对分辨率要求不高的样本。
Sepharose Fast Flow:高流速大分子分离
高度偶联的琼脂糖介质,大大加强了机械性能,流速特快,适合工业规模生产;
经去电荷处理,非特异性吸附极低,提高回收率;
极高的化学稳定性,可用多种促溶剂,有机溶剂工作及1-2M NaOH在位清洗。
Superose:分离范围最宽广
宽广的分离范围配合高分辨率,能一次性分离生物大分子大小差异大的混合物;
刚性特别好,在高粘性液体也能保持流速,适合糖类、核酸、病毒,特别是包涵体蛋白在促溶剂中的纯化。凝胶的寿命特长。
颗粒细小,大小分布集中,允许高流速纯化,适合中、高压层析系统使用
Sephacryl:经济高效、选择最多
六种不同分离范围,提供了广阔的选择性。介质反压特低、易于自行装柱;
良好的机械性能,提供快速高分辨率的纯化;
化学稳定性高过传统凝胶,可用0.5M NaOH在位清洗;
经济型Hiprerp16、26/60Sephacryl 100,200,300HR预装柱提高重复性和分辨率。
Superdex:脱盐,集团分离
经典的葡聚糖和环氧氢氯丙烷偶联介质,拥有极高的选择性;
多种分离范围、颗粒大小供选择,粗颗粒流速较快,细颗粒流速较慢,分辨率较高。
订货信息:
Sephadex
生产厂商
|
产品名称
|
规格
|
分离范围
|
货号
|
GE
|
Sephadex G-10
|
100g
|
<700
|
17-0010-01
|
GE
|
Sephadex G-10
|
500g
|
<700
|
17-0010-02
|
GE
|
Sephadex G-15
|
100g
|
100-1500
|
17-0020-01
|
GE
|
Sephadex G-15
|
500g
|
100-1500
|
17-0020-02
|
GE
|
Sephadex G-25 Coarse
|
100g
|
1000-5000
|
17-0034-01
|
GE
|
Sephadex G-25 Coarse
|
500g
|
1000-5000
|
17-0034-02
|
GE
|
Sephadex G-25 Medium
|
100g
|
1000-5000
|
17-0033-01
|
GE
|
Sephadex G-25 Medium
|
500g
|
1000-5000
|
17-0033-02
|
GE
|
Sephadex G-25 Fine
|
100g
|
1000-5000
|
17-0032-01
|
GE
|
Sephadex G-25 Fine
|
500g
|
1000-5000
|
17-0032-02
|
GE
|
Sephadex G-25 Superfine
|
100g
|
1000-5000
|
17-0031-01
|
GE
|
Sephadex G-25 Superfine
|
500g
|
1000-5000
|
17-0031-02
|
GE
|
Sephadex G-50 Coarse
|
100g
|
1000-30,000
|
17-0044-01
|
GE
|
Sephadex G-50 Coarse
|
500g
|
1000-30,000
|
17-0044-02
|
GE
|
Sephadex G-50 Medium
|
100g
|
1000-30,000
|
17-0043-01
|
GE
|
Sephadex G-50 Medium
|
500g
|
1000-30,000
|
17-0043-02
|
GE
|
Sephadex G-50 Fine
|
100g
|
1000-30,000
|
17-0042-01
|
GE
|
Sephadex G-50 Fine
|
500g
|
1000-30,000
|
17-0042-02
|
GE
|
Sephadex G-50 Superfine
|
100g
|
1000-30,000
|
17-0041-01
|
GE
|
Sephadex G-75
|
100g
|
3000-80,000
|
17-0050-01
|
GE
|
Sephadex G-75
|
500g
|
3000-80,000
|
17-0050-02
|
Sephadex LH
生产厂商
|
产品名称
|
规格
|
分离范围
|
货号
|
GE
|
Sephadex LH-20
|
25g
|
100-4000
|
17-0090-10
|
GE
|
Sephadex LH-20
|
100g
|
100-4000
|
17-0090-01
|
GE
|
Sephadex LH-20
|
500g
|
100-4000
|
17-0090-02
|
Sepharose CL
生产厂商
|
产品名称
|
规格
|
分离范围
|
货号
|
GE
|
Sepharose CL-2B
|
1 L
|
70000-40000000
|
17-0140-01
|
GE
|
Sepharose CL-2B
|
10 L
|
70000-40000000
|
17-0140-05
|
GE
|
Sepharose CL-4B
|
1 L
|
60000-20000000
|
17-0150-01
|
GE
|
Sepharose CL-4B
|
10 L
|
60000-20000000
|
17-0150-05
|
GE
|
Sepharose CL-6B
|
1 L
|
10000-4000000
|
17-0160-01
|
GE
|
Sepharose CL-6B
|
10 L
|
10000-4000000
|
17-0160-05
|
Superose
生产厂商
|
产品名称
|
规格
|
分离范围
|
货号
|
GE
|
Sepharose 2B
|
1 L
|
70000-40000000
|
17-0130-01
|
GE
|
Sepharose 2B
|
10 L
|
70000-40000000
|
17-0130-05
|
GE
|
Sepharose 4B
|
1 L
|
60000-20000000
|
17-0120-01
|
GE
|
Sepharose 4B
|
10 L
|
60000-20000000
|
17-0120-05
|
GE
|
Sepharose 6B
|
1 L
|
10000-4000000
|
17-0110-01
|
GE
|
Sepharose 6B
|
10 L
|
10000-4000000
|
17-0110-05
|
Sepharose Fast Flow
生产厂商
|
产品名称
|
规格
|
分离范围
|
货号
|
GE
|
Sepharose 4 Fast Flow
|
1 L
|
60000-20000000
|
17-0149-01
|
GE
|
Sepharose 4 Fast Flow
|
10 L
|
60000-20000000
|
17-0149-05
|
GE
|
Sepharose 6 Fast Flow
|
1 L
|
10000-4000000
|
17-0159-01
|
GE
|
Sepharose 6 Fast Flow
|
10 L
|
10000-4000000
|
17-0159-05
|
Superose
生产厂商
|
产品名称
|
规格
|
分离范围
|
货号
|
Superose 6
|
GE
|
Superose 6 10/300 GL
|
1
|
5000-5000000
|
17-5172-01
|
GE
|
Superose 6 PC 3.2/30
|
1
|
5000-5000000
|
17-0673-01
|
Superose 12
|
GE
|
Superose 12 10/300 GL
|
1
|
1000-300000
|
17-5173-01
|
GE
|
Superose 12 PC 3.2/30
|
1
|
1000-300000
|
17-0674-01
|
GE
|
Superose 6 prep grade
|
125 ml
|
5000-5000000
|
17-0489-01
|
GE
|
Superose 12 prep grade
|
125 ml
|
1000-300000
|
17-0536-01
|
Sephacryl
生产厂商
|
产品名称
|
规格
|
分离范围
|
货号
|
GE
|
Sephacryl S-100 HR
|
150 ml
|
1000-100000
|
17-0612-10
|
GE
|
Sephacryl S-100 HR
|
750 ml
|
1000-100000
|
17-0612-01
|
GE
|
HiPrep 16/60 Sephacryl S-100 HR
|
1
|
1000-100000
|
17-1165-01
|
GE
|
HiPrep 26/60 Sephacryl S-100 HR
|
1
|
1000-100000
|
17-1194-01
|
GE
|
Sephacryl S-200 HR
|
150 ml
|
5000-250000
|
17-0584-10
|
GE
|
Sephacryl S-200 HR
|
750 ml
|
5000-250000
|
17-0584-01
|
GE
|
HiPrep 16/60 Sephacryl S-200 HR
|
1
|
5000-250000
|
17-1166-01
|
GE
|
HiPrep 26/60 Sephacryl S-200 HR
|
1
|
5000-250000
|
17-1194-01
|
GE
|
Sephacryl S-300 HR
|
150 ml
|
10000-1500000
|
17-0599-10
|
GE
|
Sephacryl S-300 HR
|
750 ml
|
10000-1500000
|
17-0599-01
|
GE
|
HiPrep 16/60 Sephacryl S-300 HR
|
1
|
10000-1500000
|
17-1167-01
|
GE
|
HiPrep 26/60 Sephacryl S-300 HR
|
1
|
10000-1500000
|
17-1196-01
|
GE
|
Sephacryl S-400 HR
|
150 ml
|
20000-8000000
|
17-0609-10
|
GE
|
Sephacryl S-400 HR
|
750 ml
|
20000-8000000
|
17-0609-01
|
GE
|
Sephacryl S-500 HR
|
150 ml
|
40000-20000000
|
17-0613-10
|
GE
|
Sephacryl S-500 HR
|
750 ml
|
40000-20000000
|
17-0613-01
|
GE
|
Sephacryl S-1000 SF
|
750 ml
|
500000->100000000
|
17-0476-01
|
Superdex
生产厂商
|
产品名称
|
规格
|
分离范围
|
货号
|
GE
|
Superdex Peptide 10/300 GL
|
1
|
100-700
|
17-5176-01
|
GE
|
Superdex Peptide PC 3.2/30
|
1
|
100-700
|
17-1458-01
|
GE
|
Superdex 75 10/300 GL
|
1
|
3000-70000
|
17-5174-01
|
GE
|
Superdex 75 PC 3.2/30
|
1
|
3000-70000
|
17-0771-01
|
GE
|
Superdex 200 10/300 GL
|
1
|
10000-600000
|
17-5175-01
|
GE
|
Superdex 200 PC 3.2/30
|
1
|
10000-600000
|
17-1089-01
|
GE
|
Superdex 30 prep grade
|
25 ml
|
NMT 10000
|
17-0905-10
|
GE
|
Superdex 30 prep grade
|
150 ml
|
NMT 10000
|
17-0905-01
|
GE
|
HiLoad 16/60 Superdex 30 pg
|
1
|
NMT 10000
|
17-1139-01
|
GE
|
HiLoad 26/60 Superdex 30 pg
|
1
|
NMT 10000
|
17-1140-01
|
GE
|
Superdex 75 prep grade
|
25 ml
|
3000-70000
|
17-1044-10
|
GE
|
Superdex 75 prep grade
|
150 ml
|
3000-70000
|
17-1044-01
|
GE
|
HiLoad 16/60 Superdex 75 pg
|
1
|
3000-70000
|
17-1068-01
|
GE
|
HiLoad 26/60 Superdex 75 pg
|
1
|
3000-70000
|
17-1070-01
|
GE
|
Superdex 200 prep grade
|
25 ml
|
10000-600000
|
17-1043-10
|
GE
|
Superdex 200 prep grade
|
150 ml
|
10000-600000
|
17-1043-01
|
GE
|
HiLoad 16/60 Superdex 200 pg
|
1
|
10000-600000
|
17-1069-01
|
GE
|
HiLoad 26/60 Superdex 200 pg
|
1
|
10000-600000
|
17-1071-01
|